Functional EF-Tu with large C-terminal extensions in an E. coli strain with a precise deletion of both chromosomal tuf genes
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چکیده
منابع مشابه
an investigation of the types of text reduction in subtitling: a case study of the persian film gilaneh with english subtitles
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15 صفحه اولFunctional Constraints on Replacing an Essential Gene with Its Ancient and Modern Homologs
Genes encoding proteins that carry out essential informational tasks in the cell, in particular where multiple interaction partners are involved, are less likely to be transferable to a foreign organism. Here, we investigated the constraints on transfer of a gene encoding a highly conserved informational protein, translation elongation factor Tu (EF-Tu), by systematically replacing the endogeno...
متن کاملE. coli maltodextrin phosphorylase: primary structure and deletion mapping of the C-terminal site.
The complete 796 residue amino acid sequence of maltodextrin phosphorylase was deduced from the E. coli malP nucleotide sequence. The calculated molecular weight of 90,500, including pyridoxal phosphate, is significantly larger than experimentally determined values. Enzymatically active and inactive mutants following deletion or exchange of up to 8 codons (7 amino acids) at the 3' end (C-termin...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2003
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(03)00159-5